According to Danish Radio and Television, the three Americans traveled between Greenland and Denmark to gather information and build relationships within the framework of "secret influence activities".
The report did not name the Americans and was based on information from unnamed Danish officials.
The allegations were made by a major Danish public broadcaster on the morning of August 27. Within just a few hours, the Danish Foreign Ministry summoned the current head of the Embassy, the Interim Ambassador, to meet.
"We see that foreign forces continue to show interest in Greenland. Any attempt to interfere in the internal affairs of the royal estate is unacceptable," Danish Foreign Minister Lars Lokke Rasmussen said.
Minister Rasmussen called the summons of US diplomatic representatives a preventive discussion.
This is the second time in recent months that Denmark has summoned a US delegate on issues related to Greenland.
Earlier this year, the media reported that US intelligence agencies were tasked with collecting more information about Greenland, specifically the island's independence movement and the public's attitude towards the US exploiting resources here. Immediately after the news was published, the top US diplomat was summoned.
President Donald Trump has repeatedly expressed concern about the US taking control of Greenland, a large island of strategic importance, mainly located in the Arctic, which is Danish territory.
Trump has been pushing the idea for years, first with a proposal to buy the island from Denmark and then a statement that he would buy Greenland "one way or another." He also refused to rule out the possibility of using military force.
Denmark has repeatedly rejected President Trump's request for the US to take over Greenland.
Greenland, with a population of less than 60,000, has abundant resources, including important minerals. The island was also a base for US military operations during World War II and the Cold War. There is still a US base north of the island.
According to recent polls, the majority of Greenlanders do not want to join the US, despite many expressing their desire to separate from Denmark and become an independent nation.
According to the Danish Radio and Television report, the three Americans are trying to exploit these divisions. One person is making a list of Greenlanders who could be recruited into the breakaway movement. The remaining two are trying to build relationships with politicians, businessmen and the common people. All of this is done to undermine Greenland's ties with Denmark and bring the island closer to Washington.